點擊化學(xué)DBCO DBCO-PEG-NH2 DBCO聚乙二醇氨基 Ponsure

產(chǎn)品名稱：   二苯并環(huán)辛烯PEG氨基  DBCO-PEG-NH2                         

商品型號：   PS2-NDB

英文名：     DBCO-PEG-NH2 ,DBCO-PEG-Amine

品牌：       Ponsure

數(shù)量：     現(xiàn)貨或一周

保質(zhì)期：     一年以上

保存條件     -20℃

規(guī)格：       1g、5g、10g，大包裝另尋

詳細(xì)信息：

取代率：90%以上

分散系數(shù)：小于等于1.05

常規(guī)分子量：2K、3.4K、5K

結(jié)構(gòu)式： 

詳情介紹：

狀態(tài):淡黃色到白色固體，或者半固體，取決于分子量。

溶解性：溶于水,CHCl3，DMSO等溶劑。

保持條件：-20℃長期保存，避光，干燥。

注意事項：取用一定要干燥，避免頻繁的溶解和凍干。

應(yīng)用簡介：

Ponsure  DBCO (dibenzocyclooctyne) PEG amine is a heterobifunctional reactive PEG derivative that can go Click Chemistry reaction without a need of any metal catalysts. The strain-promoted 1,3-dipolar cycloaddition of cyclooctynes and azides, also termed as the Cu-free click reaction, is a bioorthogonal reaction that enables the conjugation of two molecules in aqueous solution. DBCO PEG derivatives possess fast kinetics and stability in aqueous buffer. DBCO reagents can be used to label azide-modified biomolecules spontaneously without the need for toxic Cu catalysts. Amine groups, on the other hand, can react with a variety of functional groups such as COOH, NHS, aldehyde, etc.

Copper-free Click reaction procedures:

Prepare the azide-containing reagents in reaction buffer.

Add PEG DBCO reagents to azide containing reagents. Add 1 mol equivalent of limiting reagent to 1.5~3.0 mol equivalents of abundance reagent.

Incubate the reaction at room temperature for 2~4 hours or 2~12 hours at 4℃.

Purify conjugates either by dialysis or size-exclusion chromatograph.

MPEG-NH2

NH2-PEG-NH2

4ARM-PEG-NH2

6ARM-PEG-NH2

8ARM-PEG-NH2

NH2-PEG-OH

BOC-NH-PEG-NH2

FMOC-NH-PEG-NH2

NH2-PEG-COOH

DSPE-PEG-NH2

DPPE-PEG-NH2

DMPE-PEG-NH2

DOPE-PEG-NH2

NH2-PEG-Tert,Amine-PEG-t-butyl acetate

NH2-PEG-SH

OPSS-PEG-NH2

DA-PEG-NH2(ACRL-PEG-NH2)

ACA-PEG-NH2

點擊化學(xué)DBCO DBCO-PEG-NH2 DBCO聚乙二醇氨基 Ponsure